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Micro and Nano Transport of Biomolecules by David Bakewell
Pankaj Yadav

Micro and Nano Transport of Biomolecules by David Bakewell

Pankaj Yadav | 08-Feb-2016 |
Introduction , Biomolecules and their electrical properties , Moving biomolecules using electric fields , Basic micro and nano-transport , Observing and quantifying and simulating electrically driven biomolecule microtransport ,

Hi friends, here Pankaj Yadav uploaded notes for Nanotechnology with title Micro and Nano Transport of Biomolecules by David Bakewell. You can download this lecture notes, ebook by clicking on the below file name or icon.

1 Introduction 8
1.1 Motivation: biomolecules in scientifi c context 8
1.2 Length scale of transport 10
1.3 Biomolecule transport example: engineered microdevices 11
1.4 Structure of this e-book 13
2 Biomolecules and their electrical properties 14
2.1 Biomolecules in cells 14
2.2 Biomolecules: structure and function 16
2.2.1 Nucleic acids 16
2.2.2 Proteins 20
2.2.3 Carbohydrates 21
2.2.4 Lipids 22
2.3 Biomolecules: electrical properties 22
2.3.1 Polyelectrolytes 22
2.3.2 DNA can be modeled as wormlike chain 24
2.3.3 Biomolecules and bioparticles 25
2.3.4 Electrical double layer 25
2.3.5 Introduction to dielectric polarization 27
2.3.6 Polarisation parameters: a brief view 28
2.3.7 Measurement of biomolecule polarisation parameters 29
2.4 Concluding remarks 30
3 Moving biomolecules using electric fi elds 31
3.1 Electrophoresis 31
3.2 Dielectrophoresis (DEP) 32
3.2.1 Polarisation and DEP biomolecule transport 36
3.2.2 Maxwell-Wagner interfacial polarisation 37
3.2.3 Maxwell-Wagner interfacial polarisation for bioparticles 38
3.2.4 Maxwell-Wagner polarisation for DNA 41
3.2.5 Counterion fl uctuation polarisation 43
3.2.6 Counterion fl uctuation polarisation for bioparticles 43
3.2.7 Counterion fl uctuation polarisation for DNA 46
3.2.8 Other polarisation mechanisms 51
3.3 Micro-environments for biomolecule transport 51
3.4 Concluding remarks 52
4 Basic micro- and nano-transport 54
4.1 Inertial, friction and sedimentation forces on single biomolecules 54
4.2 Electromagnetic forces acting on single biomolecules 56
4.2.1 Electric fi elds and electrophoresis 56
4.2.2 Inhomogenous electric fi elds and dielectrophoresis 57
4.2.3 Electroosmosis 60
4.2.4 Magnetic fi elds 60
4.3 Thermal fl uctuations 61
4.4 Combining forces for predicting single bioparticle trajectory 64
4.5 Langevin equation for a single bioparticle (biomolecule) 64
4.6 Langevin equation stochastic integration and the modifi ed diffusion equation
(MDE) 65
4.6.1 Example of one-dimensional (1D) MDE transport 67
4.6.2 1D MDE transport parameters 68
4.6.3 3D MDE transport and parameters 68
4.7 Concluding remarks 70
5 Observing, quantifying and simulating electrically driven biomolecule microtransport
71
5.1 Micro-device and experimental arrangement 71
5.2 Observations and quantitative measurements 75
5.2.1 Using geometry of DEP force aids quantifi cation 75
5.2.2 DEP collections exhibit frequency and voltage dependence 78
5.3 Simulations of electrically driven biomolecule micro-transport 80
5.3.1 Determining the dielectrophoretic force throughout the chamber. 80
5.3.2 Solutions of the MDE for predicting bioparticle collections 84
5.4 Brief discussion of experiments and theory 85
5.5 Concluding remarks 87
6 References 88
6.1 General – selected books 88
6.2 Research articles and other reading 90

 

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